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KMID : 0366119740020010013
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Korean Journal of Applied Microbiology & Bioengineering
1974 Volume.2 No. 1 p.13 ~ p.17
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Studies on the Protease produced by Streptomyces sp.
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Abstract
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A strain of Streptomyces sp. which producing a metal containing proteolytic enzyme was isolated from soil and some properties of this enzyme were investigated. The following results were obtained.
1. Optimal pH and temperature of this enzyme were pH7. 0 and 37℃
2. This enzyme was easily inactivated with heat treatment; for example, by the treatmentat 37℃ for 1.00 minutes the activity of the enzyme was decreased to about 50 per cent of intial activety but this enzyme was stable at neutral pH.
3. The activity of this enzyme was not inhibited by Mg^(++) Mn^(++), Ca^(++), Pb^(++), or Ba^(++) ect. but strongly inhibited by Hg^(+), Co^(++), Ag^(+), Cu^(++), or Cd^(++).
4. This enzyme was strongly inhibited by EDTA but was not inhibited by oxalic acid, citric acid, 2, 4-dinitrophenol, ε-aminocaproic acid, cysteine, or thiourea.
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